Etude Protek Health

Presented during BEVA Congress September 2013

Effect of Two Diets on Antioxidant Status in Racing Steeplechasers During Intensive Training

1. Van Erck E1,*, Palmers K.2, Lambey J-L.3 and Benoit S.3

 Equine Sports Medicine Practice, 83 avenue Beau Sejour, 1410 Waterloo, Belgium,
Dierenkliniek De Morette, Edingsesteenweg 237, 1730 Asse, Belgium;
SA Lambey, Moulin des prés, 71 270 Torpes, France.


High-intensity training, racing and inappropriate antioxidant supply generates high levels of deleterious oxidative stress. This
study aimed at comparing the effect of 2 commercial feeds on oxidative stress levels in steeplechasers, over a 3-month period.


A random double-blind study was undertaken to compare the effect of 2 diets in 40 racehorses. The first group received a regular
pelleted commercial diet (R) and the second group received a low-starch high-fibre diet (L). The horses were examined after a 6-week
habituation period (T0) and after 6 (T6) and 12 weeks of reinforced training (T12). Horses were raced regularly. At each step, horses
were weighed; body and clinical scores were attributed. Several blood markers were studied including vitamin A (Vit A), vitamin E
(Vit E), beta-carotene, superoxide dismutase (SOD), glutathione peroxidase (GPx), Coenzyme Q10 (CoQ10), copper (Cu), zinc (Zn),
selenium (Se) and pre- and post effort creatine kinase (CK) activity. Data were analysed using an ANOVA for repeated measurements
and a t test.


In the L-group, GPx levels were significantly higher than in the R-group at all times and a progressive and constant increase in GPx
was observed from T0 to T12. Vit E levels and CoQ10 increased at T6 in both groups but levels were significantly higher in group-L.
Selenium values were significantly higher at T0 in the L-group vs. R-group and remained stable in time in both groups. All other
markers were not significantly different between groups and did not change with time. The CK levels did not differ between groups,
however 2 horses from group-R displayed severe episodes of rhabdomyolysis.

        Conclusions and practical significance

The choice of an appropriate diet can effectively increase antioxidant protection and prevent training-induced oxidative stress
even in intensively trained racehorses during racing season.


The authors thank Mr E. Clayeux for his collaboration.
Ethical animal researchThe trainer of all horses gave informed consent for this study.
Sources of funding: Lambey SA, France.
Competing interests: J-L. Lambey is owner of Lambey SA and S. Benoit is a consultant for this feed company